Catalase+Lab+Data

Lab Questions and Marking Scheme

Catalase Lab Data

Names: Lamiah Bashoeh & Andrea Mangana
 * Factor: Temperature Above 20 degrees Celsius**
 * Variation for Temperature || Temperature Reached || Height of Foam ||
 * 20 C || 21 C || 29mL ||
 * 25 C || 26 C || 27mL ||
 * 30 C || 32 C || 22 mL ||
 * 35 C || 35 C || 17mL ||
 * 40 C || 40 C || 10 mL ||
 * F****actor: pH above 7 (basic)**
 * Variations for pH: || Total Amount || Height of Foam ||
 * 7 || 14mL || 9mL ||
 * 8 || 11mL || 6mL ||
 * 9 || 10mL || 4mL ||
 * 10 || 9mL || 3mL ||
 * 11 || 8mL || 3mL ||
 * 12 || 7mL || 2mL ||

Names: Andrew Samuel & Rafi Yama **Factor:** //Substrate Concentration//


 * __ Trial 1 __**
 * **Substrate Concentration** || **Height of foam (mL)** ||
 * 5mL of substrate & 3mL of enzyme || 18-4=14mL ||
 * 4mL of substrate, 1mL of water & 3mL of enzyme || 20-8=12mL ||
 * 3mL of substrate, 2mL of water & 3mL of enzyme || 19-8=11mL ||
 * 2mL of substrate, 3mL of water & 3mL of enzyme || 19-8=11mL ||
 * 1mL of substrate, 4mL of water & 3mL of enzyme || 12-7=5mL ||


 * Factor:** //pH below 7 (acidic)//


 * __Trial__ __1:__**
 * **pH** || **Height of Foam** ||
 * 7 || 20-6=14mL ||
 * 6( 2 drops of HCL) || 18-7=11mL ||
 * 5 (2 drops of HCL) || 16-7=9mL ||
 * 4 (2 drops of HCL) || 19-6=13mL ||
 * 3 (2 drops of HCL) || 14-7=7mL ||
 * 2 (2 drops of HCL) || 12-7=5mL ||

Name: Megan Lin & Kalle Inberg
 * Factor: Temperature 20°C and below **
 * diameter of test tube = 22.5 mm **
 * ~ __//**Trial 1**//__ || **Temperature** || **Height of foam** ||
 * ~  || 21°C || 45mm ||
 * ~  || 15°C || 47mm ||
 * ~  || 10°C || 40mm ||
 * ~  || 5°C || 31mm ||


 * ~ **//__Trial 2__//** || **Temperature** || **Height of foam** ||
 * ~  || 22°C || 43mm ||
 * ~  || 15°C || 54mm ||
 * ~  || 10°C || 45mm ||
 * ~  || 5°C || 27mm ||


 * Factor: pH 7 and up**
 * **pH** || **Volume of foam** ||
 * 8 || 12.8 mL ||
 * 8 || 4.5 mL ||
 * 9 || 9.4 mL ||
 * 10 || 7.8 mL ||
 * 11 || 6.2 mL ||
 * 12 || 5.4 mL ||

Names: Jennifer & Sharyse
 * Factor: Enzyme Concentration**
 * ~ __//Trial 1//__ || **Enzyme concentration** || **Height of Foam** ||
 * ~  || 3mL || 5mL ||
 * ~  || 2.5mL + 0.5mL of water || 3mL ||
 * ~  || 2.0mL + 1.0mL of water || 2.5mL ||
 * ~  || 1.5mL + 1.5mL of water || 2mL ||
 * || 1.0mL + 2.0mL of water || 1.7mL ||


 * ~ **//__Trail 2__//** || **Enzyme Concentration** || **Height of foam** ||
 * ~  || 3mL || 5mL ||
 * ~  || 2.5mL + 0.5mL of water || 3.5mL ||
 * ~  || 2.0mL + 1.0mL of water || 3mL ||
 * ~  || 1.5mL + 1.5mL of water || 2mL ||
 * || 1.0mL + 2.0mL of water || 1mL ||

__ Trial ____ 1: __ All have 3mL enzyme and 5mL of substrate. + 2 drops of -5 acid || 13.5mL || + 2 drops of -4 acid || 9mL || + 2 drops of -3 acid || 10mL || + 2 drops of 0.01 acid || 9mL || + 2 drops of 0.1 acid || 4mL ||
 * Factor: pH below 7 (acidic)**
 * **pH** || **Height of Foam** ||
 * 7 || 15mL ||
 * 6
 * 5
 * 4
 * 3
 * 2

__Trial 2:__ All have 3mL enzyme and 5mL of substrate + 2 drops of -5 acid || 5.5mL || + 2 drops of -4 acid || 7.5mL || + 2 drops of -3 acid || 4.5mL || + 2 drops of 0.01 acid || 5mL || + 2 drops of 0.1 acid || ||
 * **pH** || **Height of Foam** ||
 * 7 || 5mL ||
 * 6
 * 5
 * 4
 * 3
 * 2

Name: Alex Tam & Fahad Jaffar
 * Factor: Enzyme Concentration**
 * 2 min trials

//Trial 1// 3.0mL enzyme & 0mL water || 18 ||
 * **Enzyme Concentration** || **Height of Foam (mL)** ||
 * 100%
 * 83.3%2.5mL enzyme & 0.5 mL water || 6 ||
 * 66.7% 2.0mL enzyme & 1mL water || 4 ||
 * 50%1.5mL enzyme & 1**//.//**5 mL water || 5 ||
 * 33.3%1.0mL enzyme & 2mL water || 4.5 ||

//Trial 2// 3.0mL enzyme & 0mL water || 14 || 2.5mL enzyme & 0.5 mL water || 4.5 || 2.0mL enzyme & 1mL water || 4 || 1.5mL enzyme & 1.5 mL water || 5 || 1.0mL enzyme & 2mL water || 1.5 || //Trial 1// Names: Viththakan Arunthavanathan & Pratheep Rajan
 * **Enzyme Concentration** || **Height of Foam (mL)** ||
 * 100%
 * 83.3%
 * 66.7%
 * 50%
 * 33.3%
 * Variations || Volume Before || Volume After || Volume of Foam ||
 * || Foam-Liquid || Foam-Liquid || Foam ||
 * Neutral (pH 7) || 0ml – 8ml || 16ml – 5ml || 11ml ||
 * pH 8 || 0ml – 8ml || 16ml – 5.5ml || 10.5ml ||
 * pH 9 || 0ml – 8ml || 15.25ml – 6ml || 9.25ml ||
 * pH 10 || 0ml – 8ml || 14ml – 6ml || 8ml ||
 * pH 11 || 0ml – 8ml || 13ml – 7ml || 6ml ||
 * pH 12 || 0ml – 8ml || 13.5ml – 7.5ml || 6ml ||

Quantitative (for temperature 20 & below)
 * Temperature (◦C) || Trial || Time (s) || Vol. of foam || Vol. of enzyme || Vol. of substrate ||
 * 23.9 || 1 || 1:02.6 || 15.8mL || 3.0mL || 5.0mL ||
 * **22.9** || **2** || **1:00.1** || **14.6mL** || **3.0mL** || **5.0mL** ||
 * 14.9 || 1 || 0:59.9 || 6.5mL || 3.0mL || 5.0mL ||
 * **14.2** || **2** || **0:59.7** || **8.5mL** || **3.0mL** || **5.0mL** ||
 * 10.1 || 1 || 0:59.9 || 8.4mL || 3.0mL || 5.0mL ||
 * **9.9** || **2** || **1:00.7** || **7.9mL** || **3.0mL** || **5.0mL** ||
 * 6.2 || 1 || 1:00.1 || 7.1mL || 3.0mL || 5.0mL ||
 * **5.1** || **2** || **1:00.0** || **6.9mL** || **3.0mL** || **5.0mL** ||
 * Chose the ones that showed a trend and made most sense.

Quantitative (for pH 7 and above)
 * pH 7 & above || Trial || Time (s) || Vol. of foam || Vol. of enzyme || Vol. of substrate || Vol. of NaOH(aq) ||
 * 7 || 1 || 2:00.15 || 8.0mL || 3.0mL || 5.0mL || 0 drops ||
 * **7** || **2** || **1:59.80** || **14.8mL** || **3.0mL** || **5.0mL** || **0 drops** ||
 * **8** || **1** || **1:59.59** || **13.5mL** || **3.0mL** || **5.0mL** || **2 drops (0.00001)** ||
 * **9** || **1** || **2:00.01** || **11.8mL** || **3.0mL** || **5.0mL** || **2 drops (0.0001)** ||
 * **10** || **1** || **2:00.02** || **10.6mL** || **3.0mL** || **5.0mL** || **2 drops (0.001)** ||
 * **11** || **1** || **2:01.03** || **6.3mL** || **3.0mL** || **5.0mL** || **2 drops (0.01)** ||
 * **12** || **1** || **1:59.97** || **6.7mL** || **3.0mL** || **5.0mL** || **2 drops (0.1)** ||
 * Chose the ones that showed a trend and made most sense.

Names: Sunjeev and Abisheha Temperature 20°C and Above
 * **Temperature** ||  **Volume of Enzyme**  ||  **Volume of Substrate**  ||  **Volume of Foam (Best Trial)**  ||
 * 22°C || 3 mL  || 5 mL  || 24 mL  ||
 * 27°C || 3 mL  || 5 mL  || 20 mL  ||
 * 32°C || 3 mL  || 5 mL  || 18.5 mL  ||
 * 35°C || 3 mL  || 5 mL  || 17 mL  ||
 * <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">40°C || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">3 mL  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">5 mL  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">19 mL  ||

pH 7 and Below
 * **pH** ||  **Volume of Enzyme**  ||  **Volume of Substrate**  ||  **Volume of HCl(aq)**  ||  **Concentration of HCl(aq)**  ||  **Volume of Foam (Best Trial)**  ||
 * <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">2 || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">3 mL  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">5 mL  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">2 drops  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">0.1 M  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">14.5 mL  ||
 * <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">3 || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">3 mL  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">5 mL  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">2 drops  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">0.01 M  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">9.5 mL  ||
 * <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">4 || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">3 mL  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">5 mL  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">2 drops  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">0.001 M  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">8 mL  ||
 * <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">5 || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">3 mL  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">5 mL  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">2 drops  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">0.0001 M  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">7 mL  ||
 * <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">6 || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">3 mL  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">5 mL  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">2 drops  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">0.00001 M  || <span style="display: block; font-family: Calibri,sans-serif; text-align: center;">6.5 mL  ||

Names: Luxena Sribaskaran, Sahana Nirmaiyan and Lahina Sivanantharajah


 * Substrate Concentration ||  Trial 1  ||  Trial 2  ||
 * 5 mL substrate, 0 mL water (Variable 1) ||  20.73 mL  ||  19.73 mL  ||
 * 4 mL substrate, 1 mL water (Variable 2) ||  18.58 mL  ||  18.26 mL  ||
 * 3 mL substrate, 2 mL water (Variable 3) ||  11.19 mL  ||  10.95 mL  ||
 * 2 mL substrate, 3 mL water (Variable 4) ||  8.48 mL  ||  7.47 mL  ||
 * 1 mL substrate, 4 mL water (Variable 5) ||  3.96 mL  ||  3.06 mL  ||

Name: Randy and Thanusika
 * pH ||  Trial 1  ||  Trial 2  ||
 * 7 ||  3.42 mL  ||  3.23 mL  ||
 * 6 ||  2.92 mL  ||  N/A  ||
 * 5 ||  3.43 mL  ||  N/A  ||
 * 4 ||  4.61 mL  ||  N/A  ||
 * 3 ||  6.53 mL  ||  N/A  ||
 * 2 ||  4.51 mL  ||  3.91 mL  ||

Enzyme Concentration


 * Variation || Trial 1 || Trial 2 ||
 * 1 || 20 mL || 16.5 mL ||
 * 2 || 9.5 mL || 8 mL ||
 * 3 || 7 mL || 6 mL ||
 * 4 || 3 mL || 2 mL ||
 * 5 || 1.5 mL || 1.5 mL ||


 * pH || Foam ||
 * 2 || 1.5 mL ||
 * 3 || 3 mL ||
 * 4 || 3,5 mL ||
 * 5 || 4.5 mL ||
 * 6 || 5.5 mL ||
 * 7 || 5 mL ||